Fig. 3
Knockdown of A2AR expression in the CP attenuated EAE pathology. A Representative image of tdTomato flox/flox mice treated with ICV-injection of CRE-TAT. Two weeks later, the expression of tdTomato was restricted to the CP (n = 3/group). B Two weeks after ICV-injection of CRE-TAT into A2AR flox/flox mice, the expression of A2AR in the CP was notably knocked down (n = 3/group). Unpaired t test. C Specific knock-down of A2AR in the CP reduces the clinical scores (n = 5–6/group). Two-way RM ANOVA, Sidak’s multiple comparisons test. D-G Specific knock-down of A2AR in the CP reduced the expressions of ccl2, ccl5, and cxcl10 (n = 3/PBS group and n = 5/CRE-TAT group). Unpaired t test. H Specific knock-down of A2AR in the CP reduced the recruitment of CD3+ T cells to the CP at day 12 post-immunization (n = 3/group). I Quantitative analysis of leukocytes CD3+ T cells on the CP in H (n = 5/group). Unpaired t test. J The H&E staining showed that specific knock-down of A2AR in the CP attenuated the infiltration of immune cells in the CP at day 12 after immunization (n = 3/group). ###p < 0.001, *p < 0.05, **p < 0.01 and ***p < 0.001