Fig. 5

Knockdown of A_2AR expression in the CP inhibited the infiltration of Th17⁺ T cells. A Following knock-down of A_2AR signal in the CP and EAE induction, flow cytometry was performed to analyze the number of CD4⁺ or Th17⁺ T cells in the CSF, spinal cords, and spleen at day 14 post-immunization. B, C Knockdown of A_2AR expression in the CP reduced the infiltration of CD4⁺ or Th17⁺ T cells in the CSF (n = 3/group) at day 14 post-immunization. Unpaired t test. D, E Knockdown of A_2AR expression in the CP reduced the infiltration of CD4⁺ or Th17⁺ T cells in the spinal cord (n = 3/group) at day 14 post-immunization. Unpaired t test. F, G Knockdown of A_2AR expression in the CP did not alter the number of CD4⁺ or Th17⁺ T cells in the spleen (n = 3/group) at day 14 post-immunization. Unpaired t test. *p < 0.05