Fig. 6

ACJ upregulates the expression of FcεRIα in mouse TG neurons innervating conjunctiva. A Schematic diagram of ACJ model construction and workflow. B Representative gel band of FcεRIα protein in TG from control and ACJ mice. Western blot revealed a significant increase in the level of FcεRIα protein in TG. n = 4 mice/group, ***P < 0.001, ACJ vs. Control mice, Student’s t-test. C Typical microscopic images of immunofluorescence staining for FcεRIα in TG sections retrogradely labeled by conjunctival wheat germ agglutinin (WGA) from Control and ACJ mice. D ACJ upregulated FcεRIα+ among WGA-labeled conjunctiva sensory neuron. Each point represents data from one mouse, n = 5 mice/group, *P < 0.05, Student’s t-test. Scale bar: 50 μm. E–L. Representative images of TG immunofluorescence staining for FcεRIα and NF200 (E), IB4 (G), CGRP (I) and GS (K) in sections containing sensory neurons retrogradely labeled by conjunctival WGA from Control and ACJ mice. Percentage of NF200⁺ (F), IB4⁺ (H), CGRP⁺ (J), and GS⁺ (L) markers among FcεRIα⁺ conjunctiva sensory neurons in Control and ACJ mice was summarized. Each point in F, H, J, and L represented data from one mouse, n = 4 mice/group, *P < 0.05, Student’s t-test. Scale bar: 50 μm