Fig. 2

Increased Aβ oligomers and reduced plaque consolidation in microglial VPS35 deficient 5XFAD brain. a, d Representative images of Thio-S (a marker for compact fibrillar Aβ, green), OC (a marker for oligomeric Aβ, red) and 6E10 (for fibrillar Aβ) staining in the CTX (cortex) (a–c) and HIP (hippocampus) (d–f) of Ctrl:5XFAD and VPS35^CX3CR1:5XFAD male mice. High-magnification images, marked by dashed squares, were shown in right panels. Scale bars, 10 μm. (b, c, e, f) Quantifications of OC covered area (b, e) and diffuseness index (c, f) in the CTX (b, c) and HIP (e, f). The diffuseness index was defined as: (Area_6E10 − Area_Thio-S)/ Area_6E10. (n = 3 female mice per group, mean ± SD, **P < 0.01, Student’s t test)