Fig. 3

Elevated dystrophic neurites in microglial VPS35 deficient 5XFAD brain. a Representative image for ATG9A (red) and Thio-S (green) immunostaining in the CTX (cortex) and HIP (hippocampus). Scale bar = 10 μm. b, c Quantifications of Aβ associated ATG9A⁺ fluorescence density per plaque in CTX (b) and HIP (c) (n = 5 male mice per group, mean ± SD, **P < 0.01, Student’s t test). d Representative images for RTN3 (red) and Thio-S (green) immunostaining in the CTX and HIP. Scale bars, 10 μm. e, f Quantifications of Aβ associated RTN3 fluorescence density per plaque in the CTX (e) and HIP (f) (n = 5 male mice per group, mean ± SD, **P < 0.01, Student’s t test)