Fig. 7

Decreased stage 1 DAM in microglial VPS35 deficient 5XFAD brain. a Schematic diagram of Aβ associated microglia in microglial VPS35 deficient 5XFAD brain leads to the reduction in Stage1 DAM activation. b Representative images for Tmem119, ApoE with Iba1 (green) and Thio-S (gray) immunostaining. Scale bar = 10 μm. c Quantification of relative Tmem119 and ApoE fluorescence intensity in Aβ associated and Aβ un-associated microglia (n = 5 male mice per group, mean ± SD, *P < 0.05, **P < 0.01, N.S., P > 0.05, Student’s t test)