Fig. 2

Effects of TM-LeD on the number, proliferation, and activation of brain microglia in HSV-1-infected mice. Leukocytes purified from brains of WT or TM^LeD/LeD mice mock-infected (Mock) or infected with HSV-1 at the indicated dpi were assayed for markers of A microglia (CD45^intCD11b⁺), B microglia (CD45⁺CD11b⁺Tmem119⁺), C microglia undergoing proliferation (CD45⁺CD11b⁺Tmem119⁺Ki67⁺), and activated microglia, CD45^intMHC-II⁺CD11b⁺ (D) or CD45^intMHC-II⁺CD11b⁺CD86⁺ (E) by flow cytometry. The data represent means + SEM (error bars) of 4–14 samples per group. *P < 0.05; **P < 0.01; and ***P < 0.001. F Sections of brains harvested from mock-infected or infected WT or TM^LeD/LeD mice at 5dpi were subjected to immunofluorescence staining with DPAI for the nucleus and the antibody against HSV-1 or mouse Iba1. The temporal cortex region is shown. Image data are representative of at least three samples per group from two independent experiments. Scale bar, 50 μm