Fig. 1

Gene expression changes of inflammasome components in the spinal cord following sciatic nerve axotomy. a Changes in the expression of NLRP3, CASP1 and IL1B mRNAs at various time points (6 h, 1 day, 3 days, 7 days and 21 days) after nerve injury, as assessed by qPCR. N = 3 animals/group, *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA with Fisher’s LSD post hoc, compared to intact). b Representative ISH images of IL1B mRNA in spinal cord motoneurons on the contralateral and on the injured side, 1 day after axotomy. Sections were counterstained with haematoxylin. Dashed lines indicate grey-white matter transition. Arrows represent motoneurons with strong staining. c Changes in the expression of NLRP3, CASP1 and IL1B mRNAs 1 day after axotomy in animals treated with vehicle, 5-BDBD or MCC950, compared to intact animals. N = 3 animals/group, *p < 0.05, **p < 0.01, ***p < 0.001 (compared to intact), ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, n.s. non-significant (compared to ax. + vehicle) (ANOVA with Fisher’s LSD post hoc). Ax. axotomy. Mean values are shown on each bar. Bars represent average ± SEM