Fig. 4

The expression of MMP12 and pro-fibrotic markers in bone marrow-derived macrophages (BMDMs) from control and subretinal fibrosis mice. BMDMs were cultured with/without 10 ng/ml recombinant TGF-β1 for 96 h, the cells were collected for immunocytochemistry or qRT-PCR, and supernatants were used for Luminex multiplex cytokine assay. A BMDMs from normal mice were stained for MMP12 (red) and F4/80 (green), imaged by confocal microscopy. Arrows indicating MMP12^high in F4/80⁺ cells. Asterisks indicating MMP12^low in F4/80⁺ cells. B qRT-PCR analysis of Mmp12 expression in BMDMs from control and subretinal fibrosis mice (20 days after the second laser). C, D qRT-PCR analysis of fibrotic marker genes (Col1a1, Acta2, Fn1) in untreated BMDMs (C) and TGFβ1 treated BMDMs (D) from control and subretinal fibrosis mice. E, F Luminex bead-based assay of the production of pro-fibrotic cytokines by BMDMs from normal and subretinal fibrosis mice. Mean ± SD, n = 4 mice, *p < 0.05, **p < 0.01, Mann–Whitney test