Fig. 1

Modified transient transcranial MCAO (ttMCAO) mouse model. A Representative TTC stained brain (left) on day 3 after 6 h ttMCAO, and the MCA occlusion sites (right) of our model and existing models are shown. V ventral, D dorsal, R rostral, C caudal. B Functional outcome. Young mice were subjected to 6-h ttMCAO, and open field and tape removal tests were evaluated. Additional data from this experiment are shown in Additional file 1: Fig. S2. C Comparison of functional outcome between young and aged mice after ttMCAO. Young and aged mice were subjected to 6 h ttMCAO. All animals survived for 3 days. Body weight loss was evaluated on days 1 and 3 after stroke. On day 3 after stroke, mice were assessed by neurologic scoring, open field test, and tight rope test. D Time-course of immune profiling of infiltrating myeloid cells in the post-stroke young brain by flow cytometry. Young mice were subjected to 6-h ttMCAO, and immune cells in the brain were analyzed on post-stroke days 1(D1), 3 (D3), and 7 (D7). Data are presented as mean ± SEM or median (n = 5/group). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001