Fig. 7

Chronic morphine induced NLRX1-mediated immunosuppression in brain. A Confocal microscopy analysis of NLRX1 (green) and NeuN (red), CD31 (red) or GFAP (red). Bar = 50 μm. B, C, D The mRNA expression of inflammatory cytokine genes were analyzed in cortex (B), striatum (C) and cerebellum (D) in mice (n = 3–5). Quantitative graphs are shown by mean ± SEM. Data represent the mean ± SEM. Student's t-test or Mann–Whitney U test were used to measure significance between two groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and ns p > 0.05)