Fig. 5

Knockdown of LCN2 prevents microglial activation and neuroinflammation. a Representative immunofluorescence images of hippocampal sections with IbaI⁺ cells (White). Scale bars, 150 µm. b Quantification of the microglia density in (A) (n = 4 mice/group, 2–3 slides/mice). c Quantification of the microglia Ramification index at 24 h after surgery (n = 72 cells from 4 mice/group). d Number of process intersections with shells at distances (in 2 µm in increments) from the soma by Sholl analysis at 24 h after surgery (n = 72 cells from 4 mice/group). e Tissue ELISA of IL-6 at 24 h after surgery (n = 5 mice/group). f Relative expression level of tnf-α, IL-6 and/or IL-1β 6 h after application of rLCN2 (500 ng/mL) on BV-2 (n = 4–6/group) and primary microglia cells (n = 3/group). Vehicle: PBS buffer. Data represent mean ± SEM; n = 4/group. * P < 0.05; *** P < 0.001