Fig. 2

IL-13 Mφs have an anti-inflammatory phenotype and secrete functional IL-13. a–i Mφs were isolated from C57BL/6J mice. a–d Gene expression analysis by qPCR showed that IL-13 Mφs have an upregulation of Arg1 (a) and the conserved genes IRF4 (b) and KLF4 (c), while no increased expression of iNOS (d) was observed relative to M0 Mφs. Data were normalized to M0 Mφs and represent mean ± SEM. n = 8–14. e, f Western blot quantification showed increased expression of Arg1 (e) but no upregulation of iNOS (f) by IL-13 Mφs compared to M0 Mφs. Data were normalized to M0 Mφs and represent mean ± SEM. n = 4–5. g Representative immunoblot of Arg1 and iNOS expression. h, i Representative image (h) and quantification (i) of the western blot analysis of Arg1 expression. Following incubation with the conditioned medium (CM) of IL-13 Mφs, M1 Mφs showed Arg1 protein expression. Data were normalized to M1 Mφs and represent mean ± SEM. n = 3–4. Kruskal–Wallis test with Dunn’s correction. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001