Fig. 2

Transcriptional response of immune in vitro cell cultures exposed to different proinflammatory stimuli. Bulk RNA-seq was performed in different innate immune in vitro cell cultures exposed to proinflammatory stimuli during 24 h. THP-1 cells were treated with different Aβ formulations including Aβ oligomers (Ol) and Aβ fibrils (Fb) obtained using different protocols (dark blue); Aβ in the absence of FBS (turquoise), with different Aβ concentrations (light blue), or co-incubating with LPS/ INFγ and Aβ together (purple). In addition, other immune cell types were treated with Aβ (orange). Finally, THP-1 cells and IMGLs were treated with 10 ng/ml LPS + 20 ng/ml INFγ (red). Differential genes in response to Aβ or LPS/ INFγ were determined by comparing to condition-matched controls using DESeq2, p < 0.01, absolute fold change > 2