Fig. 3

Aβ is incorporated into THP-1 cells. a Aβ Oligomers and fibrils were generated using either Tseng et al. 2017 (Method a) or Abud et al. 2017 (Method b) protocol. In the last case, fibrils were generated during either 5 or 7 days of incubation (B5 and B7, respectively). 120 ng of Aβ were run in an electrophoresis on a 12% SDS-PAGE followed by western blot using anti-Aβ primary antibody (6E10 Biolegend). b THP-1 cells were labeled with CellTracker Green CMFDA dye and incubated for 1 h with 2 µg/ml of oligomeric β-Amyloid_1-42-HiLyte Fluor 555. Confocal images were acquired on an inverted Zeiss 800 laser scanning confocal microscope. Scale bar, 10 µm