Fig. 8
From: TLR4 mutation protects neurovascular function and cognitive decline in high-fat diet-fed mice
Analysis of microglia. Confocal microscopic images of cortex sections (upper panel: A–D) and hippocampus (bottom panel: L-O) with a single cell of each image cropped to show details of Sholl analysis in cortex (upper panel: E–H) and hippocampus (bottom panel: P–S). Samples marked with anti-Iba1 for microglia (yellow) and anti-IB4 (red) for vessels of wild type (WT) and TLR4-mut mice fed a normolipid diet (white bars) or a high-fat diet (black bars) for 24 weeks. The data represent the mean ± SEM of up to 40 microglial cells per group of 3 animals. Magnification 400x, scale bar 50 µm and 10 µm in depicted pictures. Graphs of the number of microglia/field I and T and Sholl analysis (J, K, U, and V) * p < 0.05 versus WT-ND, **p < 0.01 vs. WT-ND #p < 0.05 versus WT-HFD