Fig. 5
JAK–STAT signaling pathway may be involved in IL-13-induced regulation of microglial activation. A tSNE plot depicting identified CD45high brain cells obtained from 5 days after tMCAO. B Expression of microglia conserved marker in tSNE plot, P2ry12, Tmem119, Gpr34, Cx3cr1. C Left panel, dot plot showing representative homeostatic microglia markers (Cx3cr1, P2ry12, P2ry13, Gpr34, Tmem119, Selplg, Olfml3), DAM markers (Itgax, Lyz2), M1-like phenotype markers (IL-1b, S100a8, Cd74, Cd86, Ccr2, Nfkb1) and M2-like phenotype markers (Mrc1, Cd163, TGF-β). Right panel, DAM determined accordingly were highlighted in tSNE plot of microglia subclusters. D Heat map showing GSVA enrichment scores per cell of microglia subclusters in five selected KEGG signaling pathways that were significantly enriched as shown in the right bar plot which visualized the results from KEGG pathway over-representation analysis using DEGs of MG 3 versus MG1&MG2 as input. E Heat map depicting expression of the 18 DEGs from JAK–STAT signaling pathway of each cell in D and their corresponding fold change. F IL-13Rα1 expression across all clusters. G tSNE plot highlighting IL-13Rα1-expressing cells. H GSEA running score and ranked list of DEGs (IL-13Rα1+ versus IL-13Rα1−) within JAK–STAT signaling pathway