Fig. 5
Role of microglial Sirt3 in anti-inflammatory effects of IF at day 3 after ICH. A Examples of Iba-1 immunohistochemistry in the ipsilateral basal ganglia ICH, Scale bar = 50 μm. B The number of Iba-1+ cells was quantified as shown in the bar graphs. mRNA levels of IL-1β C and TNF-α D in microglia isolated from ipsilateral cerebra were measured by qRT-PCR. Secretion of IL-1β E and TNF-α F in blood after ICH was measured by ELISA. G Western blot analysis of CD16 and CCL3 protein levels in microglia isolated from ipsilateral basal ganglia and quantification of relative gray value of CD16 H and CCL3 I protein bands. Statistical analysis was performed using Student t test. Values are mean ± SD, #P < 0.05 compared with Sirt3f/f group