Fig. 2

Melatonin treatment altered intraocular Teff/Treg immune balance. A The frequency and number of eye-infiltrating CD4 + T cells were revealed by Flow cytometric analysis after melatonin treatment. B–D Flow cytometric analysis of intracellular expression of interferon gamma (IFN-γ) and interleukin (IL)-17 on CD4 + T cells harvested from the eyes of experimental autoimmune uveitis mice treated with vehicle (dimethyl sulfoxide) or melatonin on day 14 (n = 3). E–G The expression of IL-17A, IFN-γ, and Foxp3 mRNA in the retinas were measured using real-time polymerase chain reaction 14 days after immunization (n = 4). The representative values from three independent experiments. Significance was determined by unpaired t test (A–D), or one-way ANOVA (E–G). *P < 0.05, **P < 0.01, ***P < 0.001