Fig. 5

Melatonin attenuated uveitis mainly by regulating the activation and functional status of CD4 + T cells. A–C Frequencies of CD4 + PD-1 +, CD4 + CD69 + T cells, and CD44-CD62 + naïve CD4 + T cells from the spleen and DLNs of immunized mice in two groups. D Frequency and number of CD11C + cells from immunized mice in the vehicle-treated and melatonin-treated groups were measured by flow cytometry. E, F Frequency and number of CD8 + T cells, CD19 (B cells), and CD4 + T cells in immunized mice were measured by flow cytometry (four animals per group in three independently performed experiments). Significance was determined by two-way ANOVA (A–F). ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001