Fig. 2

Effects of asperosaponin VI on the morphology of microglia in hippocampus of CMS mice. A Representative micrographs of microglia in hippocampus and cortex of Ctrl or CMS mice after treatment with saline or ASA VI. Microglia were stained with Iba1 (red) by immunocytochemistry, and nuclei were stained with DAPI (blue). B–E Quantification of the area and number of Iba1⁺ cells, number and length of microglial branches in hippocampus and cortex of Ctrl or CMS mice after treatment with saline or ASA VI. Results of each group were obtained from 5 mice, and 5–6 micrographs (40 ×) containing hippocampus were collected from each sample. All Iba1⁺ cells in each micrograph were measured. Each dot in the bar graph represents the average of all Iba1⁺ cells analyzed for each mouse. F and G Levels of mRNA encoding Iba1 and CD11b in hippocampus and cortex of Ctrl or CMS mice after treatment with saline or ASA VI (n = 4 animals). Data for individual animals are displayed (mean ± SEM), *p < 0.05, **p < 0.01 (two-way ANOVA with Tukey’s multiple-comparisons test)