Fig. 2

OLDA administration ameliorates LPS-induced acute lung injury. Wild-type mice were challenged with LPS (5 mg/kg, i.t.) and immediately thereafter were treated with OLDA (5 mg/kg, i.v.) or vehicle (i.v.). A Representative H&E-stained sections of lungs (original magnification ×20) collected from mice 24 h after challenge with saline, LPS i.t. + vehicle, or LPS i.t. + OLDA. B Lung injury scores were assessed by analysis of epithelial and endothelial injury, alveolar hemorrhage, alveolar edema, PMN infiltration, and condensation. C Mice treated with OLDA had reduced leucocyte counts (CD45+ cells) in BAL fluid 24 h after induction of acute lung injury. D OLDA treatment induced IL-10 upregulation in the plasma at 2 h. E OLDA treatment induced IL-10 upregulation in BALF at 2 and 12 h (12-week-old male, n = 5 per group and per time point for all panels). *P < 0.05, **P < 0.01, ***P < 0.001, LPS-treated mice vehicle vs. OLDA, two-tailed Mann–Whitney U test at each time point