Microglial amyloid beta clearance is driven by PIEZO1 channels

Table 3 Antibodies used for immunocyto- and histochemistry

Antibody	Clone	Host	Dilution	Cat. No.	Producer	Antigen retrieval
Aβ	WO-2	MM IgG2aκ	1:1000	MABN10	Sigma	10 mM sodium citrate (pH 6.0)
CX3CR1		RbP IgG	1:100	ab8021	Abcam
GFAP	–	RhP IgG	1:500	Z0334	Dako
GFAP		ChP IgY	1:5000	ab4674	Abcam
IBA1	–	RbP IgG	1:250	019-19741	WAKO	10 mM sodium citrate (pH 6.0)
IBA1	GT10312	MM IgG1	1:250	MA5-27726	Invitrogen
P2RY12		RbP IgG	1:250	HPA14518	Sigma
PIEZO1	-	RbP IgG	1:50	PA5-77617	Invitrogen
PIEZO1	-	RbP IgG	1:500	NBP1-78446	NovusBio
PIEZO1	2–10	MM IgG	1:200	MA5-32876	Invitrogen
TMEM119		RbM IgG	1:100	ab185333	Abcam
TREM2	D8I4C	RbP IgG	1:200	91068S	Cell signaling

IgG immunoglobulin G, IgY immunoglobulin Y, MM mouse monoclonal, RbM rabbit monoclonal, RbP rabbit polyclonal, ChP chicken polyclonal

ISSN: 1742-2094