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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke

Fig. 3

Administration of anti-CXCL13 antibody reduces infarct volume and inhibits the infiltration of TFH cells into the brain following tMCAO. a Experimental design for administration of IgG2a and anti-CXCL13 antibody (100 μg) i.p. b Representative cresyl violet images to assess ischemic brain damage at 24 h tMCAO in IgG2a-treated and anti-CXCL13 antibody (100 μg)-treated mice. c Quantification of infarct volumes (mm3) at 24 h tMCAO in IgG2a-treated and CXCL13 antibody-treated mice (n = 10). d Representative flow cytometry gating of CD4+ ICOS-1+ and subsequent CXCR5+ IL-21+ cells from the ipsilateral brain of IgG2a isotype control-treated or anti-CXCL13 antibody-treated mice at 24 h following tMCAO. eg Quantification of CD4+ T cells (e), % of CD4 T cells+ for ICOS-1 (f), and % of CD4+ ICOS-1+ T cells+ for CXCR5+ and IL-21+ (g) from IgG2a isotype control-treated and anti-CXCL13 antibody-treated mice at 24 h tMCAO. (n = 6) Data are combined from three independent experiments. Data represent mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Student’s t test with Mann–Whitney U test (c, e–g)

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