Fig. 2

ITBS treatment promoted motor functional recovery in cerebral I/R injury mice after acute stroke. A Survival curve among Sham, MCAO/r and iTBS groups during 8-day observation period. No statistically significant difference in mortality data was observed between MCAO/r and iTBS groups at the end of experiment. Non-significant (ns), *P < 0.05 as determined by Log-rank (Mantel–Cox) test. B Cerebral ischemia induced higher neurological score, while iTBS failed to attenuate that. MCAO/r group displayed lower time to fall in rotarod test (C), inverted wire mesh grid grip test (D), higher laterality index in cylinder test (E), and lower distance move (F), mean velocity (G) in open filed test. ITBS significantly improved the motor behavior above. (H) Illustration of motion trajectory among Sham, MCAO/r, iTBS groups at 1 day (D1) and 9 days (D9) after stroke. I Schematic diagram of labeled footprint and footprint intensities charts. MCAO/r group displayed significant decreases in body speed (J), average speed (L), cadence (M), swing speed (P) and significant increases in duration (K), stand time of left limbs (N), swing time of right limbs (O). ITBS significantly increased body speed of right hindlimb, left forelimb (J), average speed (L), swing speed of right limb (P) and significantly reduced duration (K), stand time of left hindlimb (N), swing time of right hindlimb (P). Values are expressed as the mean ± SEM of the mean (n = 6). *P < 0.05, **P < 0.01 as determined by one-way ANOVA (Tukey’s multiple comparison test) and two-way ANOVA (Bonferroni’s multiple comparison test). RF right forelimb, RH right hindlimb, LF left forelimb, LH left hindlimb