Fig. 1

Distribution of Adrb2 in murine splenocytes and decrease of pro-inflammatory cytokines by NE in murine and human splenocyte cultures. A Cell type clusters visualized by t-SNE clustering (color coding) based on the expression of known marker genes. B t-SNE and dotplot showing Adrb2 expression per cell type. N = 2 mice. C Protein levels of TNF-α in supernatant from murine splenocytes after treatment with LPS and pretreatment with norepinephrine and/or propranolol. N = 3 mice. D Protein levels of TNF-α, IL-6 and IL-8 in supernatant from human splenocytes after treatment with LPS and pretreatment with norepinephrine and/or propranolol. N = 5 subjects. For both murine and human data, protein levels were relative compared with LPS condition. * indicates a significant difference compared to LPS treated splenocytes. Statistical differences were assessed with a one-way ANOVA and Dunnett’s multiple comparison test. t-SNE t-distributed stochastic neighbor embedding, nKT natural killer T cell, DC dendritic cell, Adrb2 adrenergic receptor β2, TNF tumor necrosis factor, IL interleukin, LPS lipopolysaccharide, NE norepinephrine