Table 1 Table of primary antibodies used in western blot method
From: Factor VII, EPCR, aPC Modulators: novel treatment for neuroinflammation
Antibody | Dilution | Company (# catalog) |
|---|---|---|
Rabbit anti-Protein C | 1:400 | Abbiotec (251142) |
Rabbit anti Thrombin | 1:400 | Bioss (19142) |
Rabbit anti-PAR1 | 1:500 | Bioss (0828R) |
Rabbit anti- TNFα | 1:500 | GeneTex (110520) |
Mouse anti-pAKT | 1:2000 | Cell Signaling (2920) |
Rabbit anti-AKT | 1:2000 | Cell Signaling (S473) |
Mouse anti-pERK | 1:10,000 | Sigma (M8159) |
Rabbit anti-ERK2 | 1:5000 | R&D Dydtems (AF1230) |