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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Activating cGAS–STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis

Fig. 1

Accumulation of double-strand DNA (dsDNA) and dynamic changes of cGAS and STING after CVST. A Typical immunofluorescent staining for dsDNA at the sham group, and 1 d and 3 d post-CVST, white arrowheads hint disintegration of the nucleus. Scale bar = 5 um. B A schematic indicating the selected brain cortex region for the whole experiment (white frame). C Representative immunofluorescent staining of cGAS and STING in the sham group and 3 d after CVST. Scale bar = 25 um. D The mRNA expression levels of the cGAS and STING post-CVST were measured by qPCR. n = 6 mice per group. **P < 0.01. E Western blot assay for the temporal profile of cGAS and STING levels from the injured cortex at sham, 6 h, 1 d, 3 d, 7 d and 14 d post-CVST. F Quantitative analysis for Western blot. n = 6 mice per group. **P < 0.01, *P < 0.05. G Representative microphotographs of immunofluorescence double staining showing the relationship between dsDNA (red) with cGAS (green) in the sham group and CVST 3 d group. Scale bar = 10 μm

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