Fig. 5

Effect of RU.521 on oxidative damage after CVST. A Typical photomicrographs of DHE staining in diverse groups. Scale bar = 50 μm. B Representative fluorescent staining of 8-OhdG in the indicated groups. Scale bar = 50 μm. C, D Quantitative analysis of different markers of oxidative damage (ROS, 8-OhdG, 3-nitrotyrosine, and MDA). Bars represent mean ± SEM. ^**P < 0.01, ^*P < 0.05. n = 4/6 per group. E Western blot assay and quantitative analysis of TXNIP in the damaged cortex post-CVST. F Immunofluorescent double staining demonstrated the enhanced 8-OhdG and cGAS exists an obvious co-localization relationship (as shown by white arrow). Scale bar = 20 μm