Fig. 4

Maternal separation (MS) alters the peripheral immune cells. A Contour plots showing inflammatory monocytes gating according to Ly6G and Ly6C expression from cells previously gated on: scatter, singlets; alive; CD45 + Lin- (CD4; CD8; Nk1.1; B220) CD11bhiCD115 + in each experimental group (see Additional file 2: Fig. S2 for complete gating strategy). B Analysis of inflammatory monocytes, C cytotoxic T cells, D dendritic CELLS, E Eosinophils, F neutrophils, G activated T helper and H activated T cytotoxic in spleen from WT and 5xFAD mice at 4 months of age (n = 5–11 animals/group). Data are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001