Fig. 5
LPS increases GAD65 expression in the hippocampus of WT but not Fkbp5-KO mice. The hippocampal tissues of WT and Fkbp5-KO mice were collected 7 days after the injection of SAL or LPS. A–F Western blotting and quantification were performed to examine the expression of NMDA receptor subunits (NR2A, B; and NR2B, C), GABAARα1 (D), GAD65 (E), and GAD67 (F). The quantification of band densities was indicated by normalizing it to the GAPDH level. qRT-PCR analysis of Gabra1 (G), Gad1 (H), and Gad2 (I) expression. LPS increased both the protein and gene expression of GAD65 in the HPC of WT mice, and these effects were reversed by the knockout of Fkbp5. Data are expressed as the mean ± SEM (n = 5 mice per group). *p < 0.05, **p < 0.01, and ***p < 0.001 for the comparison between two groups based on two-way ANOVA followed by the Tukey post hoc test