Fig. 3

Increased death of oligodendrocytes in the spinal cords of cIAP2^−/− mice. TUNEL (A) and TUNEL (Quantification: n = 7, 7). and CC1 double staining. Quantification: (n = 10, 10). B of lumbar spinal cords (L2-L4) collected at day 12 of disease. The quantification of oligodendrocyte death is shown in C. D Cytotoxicity of mixed glial cultures treated with LPS and TNFα for 24 h (n = 3, 3). E Expression of proinflammatory cytokines by cultured microglia untreated and TNFα stimulated (4 h) (n = 3, 3). C–E Mean ± SEM, *p < 0.05, T-test