Fig. 5

Increased caspase 8 expression and activation in cIAP2^−/− microglia during EAE. A Inflammatory gene expression in microglia isolated from WT and cIAP2^−/− (n = 3, 3) brains at day 12 of EAE disease. B Caspase-8 expression in naïve (n = 4, 4) and EAE (n = 4, 4) lumbar spinal cords (L2-L4, day 12 of EAE). C Caspase-8 staining of lumbar spinal (L2–L4) cords at day 12 of disease (left panels) and quantification (right panel). D Caspase-8 activity in lumbar spinal cords (L2–L4) at day 12 of disease. Caspase-8 and Iba1 E and NLRP3 and Iba1 F double staining of lumbar spinal cords (L2–L4) collected at day 12 of disease (left panels) and quantification (right panel). A–F Mean ± SEM, *p < 0.05, T test