Fig. 6

CD11c expression changes with C5a overexpression or C5aR1 ablation. A Representative images from 7 months of CD11b positive microglia in WT, Arctic, ArcticC5aR1KO, and ArcticC5a+ in the hippocampus. B–D Quantification of gene expression (TPM) of Itgam in the hippocampus (B) and percent field area of CD11b in hippocampus (C) and cortex (D) at 5, 7, and 10 months. E Representative images from 7 months of CD11c-positive microglia in WT, Arctic, ArcticC5aR1KO, and ArcticC5a+ in the hippocampus. F–H Quantification of gene expression (TPM) of Itgax in the hippocampus (F) and percent field area of CD11c in hippocampus (G) and cortex (H) at 5, 7, and 10 months. C5aR1KO and C5a+ cohorts were stained and analyzed separately. Four sections were stained per mouse and the percent field area was averaged over the 4 data points. Data shown as mean ± SEM. *p < 0.05, ^#0.1 > p > 0.05. Two-way ANOVA with Tukey’s post hoc test. N = 2–6 mice/genotype/age. Scale bar 500 µm