Fig. 5

Drp1 affects EZH2 via ROS/HIF-1α. A HIF-1α expression in RNA-Seq cerebral cortex sample of normal mice (blue, n = 3) and CKD mice (red, n = 3). B HIF-1α level in prefrontal cortical tissues of CKD mice determined by RT-qPCR. Mouse primary cortical neurons were treated with HIF-1α. C Enrichment of HIF-1α on EZH2 promoter in mouse primary cortical neurons detected by ChIP assay. D The relationship between HIF-1α and EZH2 verified by dual-luciferase reporter gene assay. E Levels of HIF-1α and EZH2 in mouse primary cortical neurons determined by RT-qPCR. Mouse primary cortical neurons were transduced with lentivirus overexpressing Drp1 and treated with antioxidant BHA. F ROS level in mouse primary cortical neurons measured by DHE staining. G Enrichment of HIF-1α on EZH2 promoter in mouse primary cortical neurons detected by ChIP assay. H The relationship between HIF-1α and EZH2 verified by dual-luciferase reporter gene assay. I EZH2 mRNA levels in mouse primary cortical neurons determined by RT-qPCR. N = 6 for mice, and n = 3 for cells. *p < 0.05