Fig. 2

Expression of C5a and C5aR in primary mouse RPE cells. Mouse RPE cells were treated with 10 ng/mL TGF-β2 for different times. A C5aR representative immunofluorescence image of RPE cells under normal conditions and after the exposure to TGF-β2 for 48 h. Scale bar = 50 µm. Arrows indicate C5aR expression. Arrowhead, spindle-shaped RPE cells. B Western blot analysis of C5aR expression in RPE cells treated with TGF-β2 for different times. Data were representative of 2–3 repeated studies and expressed as fold change of control of each time point. C, D ELISA quantification of protein concentration of C5 (C) or C5a (D) in the supernatants of control and TGF-β2-treated RPE cells. Data were normalized to supernatant total protein. Mean ± SEM. n = 3–6. *p < 0.05; ****p < 0.001, Student t-test