Fig. 3

Effect of C5a on RPE cell epithelial-to-mesenchymal transition. Primary mouse RPE cells were treated for 96 h with 50 ng/mL of recombinant C5a. Cells were collected and processed for investigations of mesenchymal and epithelial markers by Western blot (A, B) and immunocytochemistry (C). A Representative blot of α-SMA, vimentin, Slug or Fibronectin (FN), and E-cadherin (E-cad) in control and C5a-treated RPE cells. B Quantitative data of Western blot from 2 to 3 independent experiments (1 ~ 2 batches of RPE cells/experiment). Means ± SEM. *p < 0.05; **p < 0.01, Student t-test. n = 3–6. C Immunocytochemistry of α-SMA, Vimentin, FN and E-cad in control and C5a-treated RPE cells. Scale bar = 50 μm