Fig. 5

The effect of C5a on RPE wound-healing and contractility. Primary mouse RPE monolayers were subjected to a 1 mm width scratch. Cells were then exposed to C5a (50 ng/mL) for 24 h with or without PMX53 (50 nM). A Representative images from control, C5a or C5a + PMX53-treated groups immediately (0 h) and 24 h after wound scratch. B Changes in the wound area covered by the cell monolayer in different groups. Data expressed as % of remaining wound area. C Mouse RPE cells cultured in collagen gels were treated with recombinant C5a in the presence or absence of PMX53 for 24 h. The gel contraction was measured at the end of the experiment. Data expressed as % of reduction in gel area. n = 4. *p < 0.05; **p < 0.01; one-way ANOVA followed by Tukey’s multiple comparison tests. The wound scratch assay was conducted twice and the collagen gel contraction assay was conducted four times and each with different batches of pRPE cells