Fig. 7
MSCs treatment inhibit rmTBI-induced ferroptosis. A, B Western blot analysis of the Fpn, Tfr1 proteins in sham, vehicle, MSCs, Lip-1 groups. Protein expression was normalized to GAPDH and expressed as fold change of sham. C, D The levels of Fe2+ content and GPx activity in the sham, vehicle, MSCs, Lip-1 groups. E, F Western blot analysis of the 4-HNE and GPx4 proteins in sham, vehicle, MSCs, Lip-1 groups. Protein expression was normalized to GAPDH and expressed as fold change of sham. Data are expressed as mean ± SD (n = 6) and analyzed using one-way ANOVA with Tukey’s post hoc test. For vehicle group: ***P < 0.001 vs sham group; for MSCs group: #P < 0.05, ##P < 0.01, ###P < 0.001 vs vehicle group; for Lip-1 group: #P < 0.05, ##P < 0.01, ###P < 0.001 vs vehicle group