Fig. 4
From: NOX2-mediated reactive oxygen species are double-edged swords in focal cerebral ischemia in mice
Effects of apocynin on the NLRP3 inflammasome and angiogenesis. A, C Apocynin significantly increased CD31 expression on Day 3 but gradually decreased CD31 staining from 7 to 14 days following stroke compared with the vehicle. B, D Apocynin significantly reduced NLRP3 expression on Day 3 but upregulated NLRP3 levels on Days 7 and 14 compared with those in the vehicle group. A, B Representative immunofluorescence images of CD31 and NLRP3 staining and DAPI counterstaining at 3, 7 and 14 days after stroke in the ischemic border. C, D Quantification of CD31- and NLRP3-positive cells in the ischemic border. n = 5/group. Scale bar = 50 μm. *P < 0.05 vs. the vehicle group. All data are expressed as the mean ± SD and were analyzed by one-way ANOVA with Bonferroni's post hoc test