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Table 1 The 26 measurements that are used to characterize the microglial morphology

From: Ablation of Siglec-E augments brain inflammation and ischemic injury

Measurement

Definition

Kurtosis

The fourth-order moment about the mean

Min. gray level

Minimum gray values within the selection

Median

The median value of the pixels in the image or selection

Modal gray value

Most frequently occurring gray value within the selection

Mean gray value

Average gray value within the selection

Circularity

 = 4π × area/perimeter2

Solidity

 = Area/convex area

Max. gray level

Maximum gray values within the selection

Roundness

 = 4 × area/(π × major_axis2), or the inverse of the aspect ratio

Average branch length

The average length of branches, in the corresponding units

Aspect ratio

 = major_axis/minor_axis

Standard deviation

Standard deviation of the gray values

Maximum branch length

The maximum length of branches, in the corresponding units

MinFeret

The minimum caliper diameter

Feret’s diameter

The longest distance between any two points along the selection boundary

Integrated density

The product of area and mean gray value

Perimeter

The length of the outside boundary of the selection

End-point pixels

Count of pixels if they have less than 2 neighbors

Area

Area of selection in square pixels

Skewness

The third order moment about the mean

Branches

The number of branches

Slab pixels

Count of pixels if they have exactly 2 neighbors

Quadruple points

The number of triple points

Triple points

The number of quadruple points

Junctions

The number of actual junctions

Junction pixels

Count of pixels if they have more than 2 neighbors

  1. More detailed information about these measurements can be found from https://imagej.nih.gov/ij/docs/menus/analyze.html and [49]