Fig. 2

The effect of ASK1 cKO on acute and chronic seizures after KA injection. A ASK1 knockout in mice did not affect weight. B, C ASK1 cKO downregulated the frequency of acute seizures (B), but did not affect the average seizure score C in the acute period. D The number of seizures in the acute period in the WT KA and cKO KA groups is displayed in the form of a heatmap, with a darker color indicating more seizures. E–F ASK1- cKO reduced the total number of SRSs (E), but the average seizure score F was not affected by ASK1 cKO. G The number of SRSs in the WT KA and cKO KA groups for 8 consecutive days (Day 25 to Day 32 after KA injection) is displayed in the form of a heatmap, with a darker color indicating more SRSs. All data are expressed as the mean ± SD. ***p < 0.001, *p < 0.05, WT KA group vs. WT Sham group, or as indicated; ^##p < 0.01, and ^#p < 0.05, cKO KA group vs. cKO sham group. n = 8 in the WT sham or cKO sham groups, n = 12 in WT KA or cKO KA groups, p values are based on the two way ANOVA test (A). n = 18 in WT KA and cKO KA groups in the acute period (B–D), and n = 12 in WT KA and cKO KA group in the chronic period (E–G), p values are based on the Student’s t test or Mann–Whitney U test (B–F)