Fig. 6

Vitreoretinal interface macrophages include mostly microglia with minor numbers of perivascular macrophages and hyalocytes. A Confocal immunofluorescence merged image with CD31 (blue), GFP (green), IBA1 (magenta), and CD169 (red) of a Tmem119^GFP/+ retinal flatmount centered at the optic nerve. The highlighted box shows the area magnified in B–E. Magnified merged (B), GFP/CD31 (C), CD169/CD31 (D), and IBA1/CD31 (E) image. White arrows highlight IBA1⁺GFP^negCD169⁺ hyalocytes and the yellow arrowheads highlight IBA1⁺GFP^negCD169^neg perivascular macrophages (PV-Mac). Retinal surface macrophages are composed of 82% IBA1⁺GFP⁺CD169^neg microglia, 8% IBA1⁺GFP^negCD169^neg perivascular macrophages, and 8% IBA1⁺GFP^negCD169⁺ hyalocytes (F, *p < 0.05, **p < 0.01, repeated measures analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test). N = 3 mice from a single litter that were quantitated. The experiment was repeated independently but not quantitated