Skip to main content

Table 1 Immunofluorescence imaging antibodies

From: Macrophages in close proximity to the vitreoretinal interface are potential biomarkers of inflammation during retinal vascular disease

Antibody

Fluorophore

Dilution

Manufacturer, product number

Goat anti-mouse CD31

–

1:250

R&D Systems, AF3628

Rabbit anti-mouse IBA1

–

1:500

Wako, 019–19,741

Chicken anti-mouse GFP

–

1:6000

Abcam, ab13970

Rat anti-mouse ICAM2

–

1:500

BD Biosciences, 553,326

Rabbit anti-mouse TMEM119

–

1:1000

Synaptic Systems, 400 002

Rat anti-mouse CD169

–

1:200

Bio-Rad, MCA884

Rat anti-mouse Ly6C

–

1:1000

Abcam, ab54223

Rat anti-mouse CD206

–

1:200

Bio-Rad, MCA2235

Rabbit anti-Collagen IV

–

1:500

Abcam, 19,808

Donkey anti-goat (H + L)

Alexa Fluor 405

1:500

Invitrogen, A48259

Donkey anti-rabbit (H + L)

Alexa Fluor 647

1:500

Invitrogen, A31573

Donkey anti-chicken (H + L)

Alexa Fluor 488

1:500

Jackson ImmunoResearch, 703–545-155

Donkey anti-rat (H + L)

TRITC

1:500

Invitrogen, A18750