Fig. 3

HDAC3 miKO alleviates neuroinflammation after TBI without altering infiltrating blood immune cells. HDAC3 miKO mice and WT control mice were subjected to TBI induced by CCI, and brain inflammation profiles were examined 5 days after TBI. A, B A panel of 40 inflammatory cytokines was measured in the ipsilesional brain hemisphere using an antibody array. A Representative blots with significantly altered markers labeled. B Summarized data showing the mean expression levels of 14 markers that were significantly different among groups. Non-injured baseline controls were pooled from both WT and HDAC3 miKO mice, between which there was no significant difference (see Additional file 1: Fig. S1). C, D Infiltration of peripheral immune cells into the post-TBI brain was assessed using flow cytometry 5 days after TBI. C Flow cytometry gating strategy for various immune cells in the brain. D Summarized data showing the numbers of immune cells in the ipsilesional brain hemisphere, expressed as fold changes over the non-injured contralesional side. Shown are the mean ± SD. n = 6–7 mice per group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 TBI vs. baseline control. *p < 0.05, ***p < 0.001 HDAC3 miKO vs. WT after TBI. ns no significant difference