Fig. 7
From: Sensory neuron dysfunction in orthotopic mouse models of colon cancer
Bulk RNA sequencing of DRGs from MC38-injected mice. Bulk RNA sequencing analysis on lumbar DRGs of mice collected 3 weeks after tumor inoculation (n = 3/group). a Volcano plot showing the different genes with significantly decreased (blue) or increased (red) expression (p≤ 0.05). b Gene expression pathway enrichment analysis (MC38-injected vs vehicle-injected mice). Blue: immune/inflammation-related pathways; grey: hemostasis/clotting/thrombotic pathways (consistent with elevation of serpin E1 and chitinase-3 like 1, among others, in the proteome profiler arrays). c Immunohistochemistry of lumbar DRG from vehicle-injected and MC38 tumor-bearing mice at 3 weeks indicates CXCL10 immunoreactivity (green) overlaps with the neuronal marker NF200 (red), as well as a subpopulation of small–medium diameter neurons. DAPI: blue, scale bar = 100 μm