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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Transcriptome profiling of microRNAs reveals potential mechanisms of manual therapy alleviating neuropathic pain through microRNA-547-3p-mediated Map4k4/NF-κb signaling pathway

Fig. 5

Validation of Map4k4 as a target gene of miR-547-3p. A Prediction of binding sites between miR-547-3p and Map4k4. B Luciferase reporter gene assay was performed to validate the interaction between miR-547-3p and Map4k4. The data are expressed as mean ± SD (N = 3) and analyzed for statistical significance using one-way ANOVA followed by LSD post hoc analysis for multiple comparisons (**P < 0.01 versus miRNA-NC + Map4k4-WT group). C, D Western blot and qRT-PCR analysis show the effects of miR-547-3p mimics or inhibitor on the expression of Map4k4 in the DRG cells. The data are expressed as mean ± SD (N = 3) and analyzed for statistical significance using one-way ANOVA followed by LSD post hoc analysis for multiple comparisons (**P < 0.01 versus control group). DRG dorsal root ganglion, LSD least significant difference, NC negative control, WT wild-type, MUT mutant, qRT-PCR real-time quantitative reverse transcription PCR, SD standard deviation

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Journal of Neuroinflammation

ISSN: 1742-2094