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Table 1 Primer sequences used for qRT-PCR analysis

From: Transcriptome profiling of microRNAs reveals potential mechanisms of manual therapy alleviating neuropathic pain through microRNA-547-3p-mediated Map4k4/NF-κb signaling pathway

Gene name

Primer sequence (5′–3′)

miR-375-3p

RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGATCACGC

 

Forward: GCGCTTTGTTCGTTCGGCTC

 

Reverse: TGCAGGGTCCGAGGTAT

miR-449a-5p

RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGAACCAGC

 

Forward: GCGCTGGCAGTGTATTGTTA

 

Reverse: TGCAGGGTCCGAGGTAT

miR-547-3p

RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGATCTCAC

 

Forward: GCGCATTGGTACTTCTTTAA

 

Reverse: TGCAGGGTCCGAGGTAT

U6

Forward: CTCGCTTCGGCAGCACA

 

Reverse: AACGCTTCACGAATTTGCGT

Map4k4

Forward: GTCCTGTCCCGTCGAGATTC

 

Reverse: GCCAACGCAGTCAAGTCAAT

p65

Forward: GCTTCATATGCGGGCAACAG

 

Reverse: AAGCAATGAGCCACTCCCTC

IκBα

Forward: AGACTCGTTCCTGCACTTGG

 

Reverse: TCTCGGAGCTCAGGATCACA

GAPDH

Forward: AGGTGACCGCATCTTCTTGT

 

Reverse: TACGGCCAAATCCGTTCACA

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Journal of Neuroinflammation

ISSN: 1742-2094