Fig. 4

RS102895 alleviated CPS-induced depressive-like behaviors by inhibiting the infiltration of BM-derived monocytes into the hippocampus. A Schematic of the experimental schedule. After successful BMT, mice received the treatment of RS102895 or vehicle from 1 day before CPS induction until the end of CPS exposure. Finally, the mice underwent behavioral tests. B Representative image of BM-derived GFP⁺ cells (green) in the hippocampus (n = 4 mice/group). RS102895 suppressed the infiltration of BM-derived GFP⁺ cells into the hippocampus. Scale bars, 200 μm. C Quantification of GFP⁺ cells recruited to the hippocampus (n = 4 mice/group, Kruskal–Wallis one-way ANOVA, P = 0.020). Graphical data are represented as box and whisker plots. The box shows the lower, median and upper quartiles, and the whisker shows the minimum and maximum values. D Representative trajectories of indicated mice in OFT (n = 12 mice/group). E Total distance traveled within each 5-min period in indicated groups in OFT (n = 12 mice/group, one-way ANOVA, F_{(2, 33)} = 0.762, P = 0.475). F Time spent in the central zone in indicated groups in OFT (n = 12 mice/group, one-way ANOVA, F_{(2, 33)} = 4.960, P = 0.013). G Immobility time of mice in indicated groups in FST (n = 12 mice/group, one-way ANOVA, F_{(2, 33)} = 10.093, P < 0.001). H Sucrose consumption of mice in indicated groups in SCT (n = 12 mice/group, one-way ANOVA, F_{(2, 33)} = 9.933, P < 0.001). *P < 0.05, ***P < 0.001. ns, not significance. Error bars represent SEM