Fig. 1
Human astrocytes exposed to IL-27 and/or IL-1β show differential gene expression associated with immune functions. Gene expression profiles of human astrocytes either untreated (Untr) or IL-1β-inflamed (Inf) and then exposed or not to IL-27 (Untr + IL-27; and Inf + IL-27) for 24 h were analyzed. n = 4 individual donors. A Principal component analysis (PCA) of the transcriptomic profile of Untr (pink), Untr + IL-27 (green), Inf (yellow), and Inf + IL-27 (brown). B Volcano plots showing the number of upregulated (red) and downregulated (blue) genes in Untr. vs. IL-27-exposed, untreated vs. inflamed (IL-1β-exposed), inflamed (IL-1β-exposed) vs. inflamed + IL-27 astrocytes, and untreated-IL-27 vs. IL-1β + IL-27. C Heatmap of the upregulated genes induced by IL-27 in both non-inflamed and inflamed astrocytes. DEGs and samples are ordered according to hierarchical clustering within the space of the displayed DEGs (pheatmap package). Each individual donor is color-coded (donors 1 to 4). D Venn diagram presenting the number of IL-27-up-regulated genes in either untreated (Untr) or inflamed (Inf) astrocytes or shared by both conditions. Examples of genes are indicated. E, F Gene Set Enrichment Analysis (GSEA) of the upregulated genes induced by IL-27 E or IL-1β. F Exposed astrocytes. Genes were ranked according to their t-statistic for the corresponding comparisons. The log2 of the odd ratio (logOR) is presented on the x-axis and the –log10 of the adjusted P value (-log10(adjusted P-val)) is represented with the color legend. GSEA shared between IL-27 and IL-1β are shown in blue